These problems are alleviated by the addition of appropriate enzymes such as endo-xylanase and β-glucanase to the feed.
The major carbohydrate, anti-nutritional factors in legume seeds are the non-digestible raffinose-series-oligosaccharides. These can be converted to digestible sugars by α-galactosidase treatment. Megazyme has recognised the need for simple and specific assays for these anti-nutritional carbohydrates, and has developed the required test kits.
Analyte | Cat. No. | Analyte Significance | Advantages of Megazyme Test Kits |
---|---|---|---|
Acetic Acid | K-ACETRM | Commonly found in feed or fermented feed | K-ACETRM is a rapid, manual assay kit employing AK and phosphotransacetylase. Stable reagents |
Ammonia | K-AMIAR | Commonly found in feed or fermented feed | Rapid reaction rate (~ 3 min at room temperature). Ideal for manual and auto-analyser applications. Stable reagents |
α-Amylase |
K-CERA |
A key enzyme in most feeds and plant products | Novel assay employing a defined oligosaccharide substrate. High sensitivity and specificity. AOAC Method 2002.01; AACC Method 22-02.01; ICC Standard No. 303; RACI Standard Method; CCFRA Flour Testing Working Group Method 0018 |
Available Carbohydrates / Dietary Fiber | K-ACHDF | Rapidly and slowly available sugars for digestion or fermentation | Novel procedure, stable reagents |
Fructan | K-FRUC | Fructo-oligosaccharides in grasses and grains | Only kit available. Stable reagents; AOAC Method 999.03; AACC Method 32-32.01 |
D-Fructose / D-Glucose |
K-FRUGL K-FRGLMQ K-FRGLQR |
Major digestible carbohydrates in feeds | Rapid reaction times, choice of simple formats available, ideal for manual and auto-analyser applications, stable reagents |
Galactomannan | K-GALM | Reserve carbohydrate in many legume seeds | Only kit available, stable reagents |
β-Glucan (Barley and oats) | K-BGLU | Major cell-wall polysaccharide of barley and oats | Rapid reaction, stable reagents, only enzymatic kit available. AOAC Method 995.16; AACC Method 32-23.01; EBC Methods 3.11.1, 4.16.1 and 8.11.1; ICC Standard No.166; RACI Standard Method |
β-Glucanase | K-CELLG3 | β-Glucanase in feed | Novel assay employing a defined oligosaccharide substrate. High sensitivity, specificity and stability. Rapid reaction, ideal for manual and auto-analyser applications |
β-Glucanase | K-MBGL | Cellulase and β-glucanase levels in feeds | Only kit available. Stable reagents. RACI Standard Method |
L-Lactic Acid | K-LATE | Commonly found in fermented feed | Rapid reaction, stable reagents. Ideal for manual and auto-analyser applications |
Phytic Acid | K-PHYT | Found in most plant materials. Major form of bound phosphate in plant materials | Novel procedure. Rapid reaction, stable reagents |
Raffinose / D-Galactose | K-RAFGA | Found in high levels in legume seeds. Causes discomfort and flatulence in pigs | Rapid reaction, stable reagents |
Resistant Starch | K-RSTAR | Starch that is not digested in the small intestine of monogastric animals | Only kit available. Stable reagents AOAC Method 2002.02; AACC Method 32-40.01 |
Total Dietary Fiber |
K-TDFR K-INTDF |
Carbohydrate not digested in small intestine |
1. K-TDFR: AOAC Methods 985.29, 991.42, 991.43 & 993.19; AACC Methods 32-05.01, 32-06.01, 32-07.01 & 32-21.01. 2. K-INTDF is consistent with the CODEX Alimentarius definition of dietary fiber. AOAC Methods 2009.01 & 2011.25; AAAC Methods 32-45.01 & 32-50.11 |
Total Starch |
K-TSTA K-TSHK |
Starch content of grain and feed | Rapid assay formats with options of measuring D-glucose with GOPOD reagent or with hexokinase / G-6-PDH. Stable reagents. AOAC Method 996.11; AACC Method 76-13.01; ICC Standard No. 168; RACI Standard Method |
endo-β-Xylanase | K-XYLS | β-Xylanase in feed | High sensitivity. Stable reagents |
Beta-Glucanase | S-ABG100 | b-Glucanase in feed | Rapid reaction. Sensitive. Stable reagent |
endo-β-Xylanase | S-AXBP | b-Xylanase in feed | Sensitive. Easy to use. Stable reagent |
Protease | S-AZCAS | endo-Protease added to feed | Easy to use. Stable reagent |