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Анализ и тестирование кормов для животных

Cereal grains and legume seeds are major components in the feed formulations for chickens and pigs. These materials contain a range of anti-nutritional factors such as non-digestible polysaccharides and oligosaccharides. Arabinoxylan in wheat and β-glucan in barley increase the viscosity of the gut content and reduce the rate of feed consumption and nutrient absorption.

These problems are alleviated by the addition of appropriate enzymes such as endo-xylanase and β-glucanase to the feed.

The major carbohydrate, anti-nutritional factors in legume seeds are the non-digestible raffinose-series-oligosaccharides. These can be converted to digestible sugars by α-galactosidase treatment. Megazyme has recognised the need for simple and specific assays for these anti-nutritional carbohydrates, and has developed the required test kits.

Analyte	Cat. No.	Analyte Significance	Advantages of Megazyme Test Kits
Acetic Acid	K-ACETRM	Commonly found in feed or fermented feed	K-ACETRM is a rapid, manual assay kit employing AK and phosphotransacetylase. Stable reagents
Ammonia	K-AMIAR	Commonly found in feed or fermented feed	Rapid reaction rate (~ 3 min at room temperature). Ideal for manual and auto-analyser applications. Stable reagents
α-Amylase	K-CERA	A key enzyme in most feeds and plant products	Novel assay employing a defined oligosaccharide substrate. High sensitivity and specificity. AOAC Method 2002.01; AACC Method 22-02.01; ICC Standard No. 303; RACI Standard Method; CCFRA Flour Testing Working Group Method 0018
Available Carbohydrates / Dietary Fiber	K-ACHDF	Rapidly and slowly available sugars for digestion or fermentation	Novel procedure, stable reagents
Fructan	K-FRUC	Fructo-oligosaccharides in grasses and grains	Only kit available. Stable reagents; AOAC Method 999.03; AACC Method 32-32.01
D-Fructose / D-Glucose	K-FRUGL K-FRGLMQ K-FRGLQR	Major digestible carbohydrates in feeds	Rapid reaction times, choice of simple formats available, ideal for manual and auto-analyser applications, stable reagents
Galactomannan	K-GALM	Reserve carbohydrate in many legume seeds	Only kit available, stable reagents
β-Glucan (Barley and oats)	K-BGLU	Major cell-wall polysaccharide of barley and oats	Rapid reaction, stable reagents, only enzymatic kit available. AOAC Method 995.16; AACC Method 32-23.01; EBC Methods 3.11.1, 4.16.1 and 8.11.1; ICC Standard No.166; RACI Standard Method
β-Glucanase	K-CELLG3	β-Glucanase in feed	Novel assay employing a defined oligosaccharide substrate. High sensitivity, specificity and stability. Rapid reaction, ideal for manual and auto-analyser applications
β-Glucanase	K-MBGL	Cellulase and β-glucanase levels in feeds	Only kit available. Stable reagents. RACI Standard Method
L-Lactic Acid	K-LATE	Commonly found in fermented feed	Rapid reaction, stable reagents. Ideal for manual and auto-analyser applications
Phytic Acid	K-PHYT	Found in most plant materials. Major form of bound phosphate in plant materials	Novel procedure. Rapid reaction, stable reagents
Raffinose / D-Galactose	K-RAFGA	Found in high levels in legume seeds. Causes discomfort and flatulence in pigs	Rapid reaction, stable reagents
Resistant Starch	K-RSTAR	Starch that is not digested in the small intestine of monogastric animals	Only kit available. Stable reagents AOAC Method 2002.02; AACC Method 32-40.01
Total Dietary Fiber	K-TDFR K-INTDF	Carbohydrate not digested in small intestine	1. K-TDFR: AOAC Methods 985.29, 991.42, 991.43 & 993.19; AACC Methods 32-05.01, 32-06.01, 32-07.01 & 32-21.01. 2. K-INTDF is consistent with the CODEX Alimentarius definition of dietary fiber. AOAC Methods 2009.01 & 2011.25; AAAC Methods 32-45.01 & 32-50.11
Total Starch	K-TSTA K-TSHK	Starch content of grain and feed	Rapid assay formats with options of measuring D-glucose with GOPOD reagent or with hexokinase / G-6-PDH. Stable reagents. AOAC Method 996.11; AACC Method 76-13.01; ICC Standard No. 168; RACI Standard Method
endo-β-Xylanase	K-XYLS	β-Xylanase in feed	High sensitivity. Stable reagents
Beta-Glucanase	S-ABG100	b-Glucanase in feed	Rapid reaction. Sensitive. Stable reagent
endo-β-Xylanase	S-AXBP	b-Xylanase in feed	Sensitive. Easy to use. Stable reagent
Protease	S-AZCAS	endo-Protease added to feed	Easy to use. Stable reagent